detection of mycoplasma synoviae infection in broiler breeder farms of tehran province using pcr and culture methods

Authors

f. elhamnia

m. banani

g.r. shokri

s.a. pourbakhsh

a. ashtari

abstract

mycoplasma synoviae (ms) is an important avian pathogen that can cause both respiratory disease and joint inflammation synovitis in poultry, inducing economic losses to the iranian chicken industry especially breeder farms. the aim of this study was to use the ms specific pcr and culture methods in order to detect of m. synoviae from breeder farms where located in tehran province. a total of 475 samples including choanal cleft, trachea, ovary and /or joint cavities from 23 broiler breeder farms of tehran area were collected. samples were cultured in pplo broth media supplemented for ms isolation. the bacteria dnas were extracted by phenol/chloroform method. specific published primers amplify a 207 bp region of the 16s rrna gene of ms were used for pcr method. out of 475 samples, 146 cultures were shown positive and typical mycoplasma colonies, 85 samples were also identified ms based on agglutination test with specific ms antiserum and the pcr method. a total of 122 samples, a band with 207 bp was shown as ms specific pcr product in electrophoresis. in addition to these 85 samples that were positives in both culture and pcr, 37 samples that had not grown in mycoplasma media were positive in ms specific pcr. a total of 292 samples were negatives in both culture and pcr methods. 122 positive samples out of 475 samples (25.7%) were belonged to 7 breeder farms (30.4%). on conclusions, the ms infection of broiler breeder farms of tehran area was confirmed truly. from the results, as the pcr method reduces the time consuming, an effectiveness and efficient for detection of m. synoviae infection of chicken breeder. it is then suggested that the pcr method could be an alternative method for culturing.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Detection of Mycoplasma synoviae infection in broiler breeder farms of Tehran province using PCR and culture methods

Mycoplasma synoviae (MS) is an important avian pathogen that can cause both respiratory disease and joint inflammation synovitis in poultry, inducing economic losses to the Iranian chicken industry especially breeder farms. The aim of this study was to use the MS specific PCR and culture methods in order to detect of M. synoviae from breeder farms where located in Tehran province. A total of 47...

full text

Application of culture and polymerase chain reaction (PCR) methods for isolation and identification of Mycoplasma synoviae on broiler chicken farms

Mycoplasma synoviae (M. synoviae) is a major worldwide poultry pathogen that causes serious economic losses in the poultry industry. This study was designed to detect M. synoviae through culture isolation and polymerase chain reaction (PCR) assay to demonstrated the involvement of M. synoviae infection in trachea and the lung/air sac samples taken from commercial broiler chicken farms in 3 m...

full text

Incidence and risk factors of Mycoplasma synoviae infection in broiler breeder farms of Iran

Mycoplasma synoviae (MS) is an important pathogen of poultry worldwide, causing respiratory tract infection and infectious synovitis in chickens and turkeys. The aim of this study was to determine the incidence and risk factors (age, size of flock, locale, sex and strain) of Mycoplasma synoviae in broiler breeder farms in Iran. The study was based on Rapid Serum Plate Agglutination (SPA) and en...

full text

application of culture and polymerase chain reaction (pcr) methods for isolation and identification of mycoplasma synoviae on broiler chicken farms

mycoplasma synoviae (m. synoviae) is a major worldwide poultry pathogen that causes serious economic losses in the poultry industry. this study was designed to detect m. synoviae through culture isolation and polymerase chain reaction (pcr) assay to demonstrated the involvement of m. synoviae infection in trachea and the lung/air sac samples taken from commercial broiler chicken farms in 3 m...

full text

Detection of Mycoplasma gallisepticum and Mycoplasma synoviae among Commercial Poultry in Khouzestan Province, Iran

Mycoplasmas are important  avian  pathogens,  which  can  cause  both  respiratory  disease  and  synovitis  in  poultry  that  result  in  considerable  economic  losses  to  the  poultry  industry  all  over  the  world.  The  aim  of  this  study  was  to  determine  the  prevalence  of  Mycoplasma  gallisepticum  and  Mycoplasma  synoviae  infections  among  commercial  poultry  flocks  in ...

full text

investigation of single-user and multi-user detection methods in mc-cdma systems and comparison of their performances

در این پایان نامه به بررسی روش های آشکارسازی در سیستم های mc-cdma می پردازیم. با توجه به ماهیت آشکارسازی در این سیستم ها، تکنیک های آشکارسازی را می توان به دو دسته ی اصلی تقسیم نمود: آشکارسازی سیگنال ارسالی یک کاربر مطلوب بدون در نظر گرفتن اطلاعاتی در مورد سایر کاربران تداخل کننده که از آن ها به عنوان آشکارساز های تک کاربره یاد می شود و همچنین آشکارسازی سیگنال ارسالی همه ی کاربران فعال موجود در...

My Resources

Save resource for easier access later


Journal title:
archives of razi institute

Publisher: razi vaccine & serum research institute (rvsri)

ISSN 0365-3439

volume 65

issue 2 2016

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023